{"id":406,"date":"2013-04-17T14:52:42","date_gmt":"2013-04-17T14:52:42","guid":{"rendered":"http:\/\/people.utm.my\/firdausw\/?p=406"},"modified":"2014-01-27T10:50:26","modified_gmt":"2014-01-27T02:50:26","slug":"mscphd-positions-available","status":"publish","type":"post","link":"https:\/\/people.utm.my\/firdausw\/2013\/04\/17\/mscphd-positions-available\/","title":{"rendered":"MSc\/PhD positions available"},"content":{"rendered":"<p>MSc\/PhD positions are immediately available on the project entitled &#8220;<strong>Elucidation of the Human Heat Shock Protein 47 (HSP47) pH-Triggered Release from Collagen via Mutational Analysis<\/strong>&#8220;.<\/p>\n<p>This project involves the mutagenesis of mouse\/human HSP47 protein towards the elucidation of its binding and release behaviour to collagen. HSP47 is a molecular chaperone that assists collagen folding and transport during biosynthesis in the cell. Knocking out of the mouse <em>hsp47<\/em> gene has been shown to lethally impair the development of embryos, preventing the secretion of correctly folded triple helical collagen molecules. Mutations in human <em>hsp47<\/em> gene has been reported to be related to the severe form of osteogenesis imperfecta, a disease characterised by bone fragility and fractures that may be accompanied by bone deformity, dentinogenesis imperfecta, short stature, and shortened life span. HSP47 is also a potential target molecule for treatment of collagen-related diseases, such as fibrosis. In order to assist research in treatment strategies, the mechanistic behaviour of HSP47 must be completely solved first.<\/p>\n<p>This project will involve site-directed mutagenesis to investigate HSP47 binding-and-release behaviour to collagen at the molecular level. Mutated proteins will be analysed using a number of techniques, such as binding assay, fluorescence-based pH titration, circular dichroism and collagen antifibrillisation assay.<\/p>\n<p>Candidates are expected to have a strong background in molecular biology and biochemistry, particularly protein expression, purification, and analysis. Graduates with good BSc degree (CPA &gt;3.00) are encouraged to apply.\u00a0<em>Scholarship is available for Malaysian students only.<\/em><\/p>\n<p>Please contact <strong>Dr Mohd Firdaus Abdul Wahab<\/strong> (email <strong>firdausw@utm.my<\/strong> or office <strong>+607-555 7551<\/strong>).<\/p>\n","protected":false},"excerpt":{"rendered":"<p>MSc\/PhD positions are immediately available on the project entitled &#8220;Elucidation of the Human Heat Shock Protein 47 (HSP47) pH-Triggered Release from Collagen via Mutational Analysis&#8220;. This project involves the mutagenesis of mouse\/human HSP47 protein towards the elucidation of its binding and release behaviour to collagen. HSP47 is a molecular chaperone that assists collagen folding and [&hellip;]<\/p>\n","protected":false},"author":5588,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[],"class_list":["post-406","post","type-post","status-publish","format-standard","hentry","category-uncategorized"],"_links":{"self":[{"href":"https:\/\/people.utm.my\/firdausw\/wp-json\/wp\/v2\/posts\/406","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/people.utm.my\/firdausw\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/people.utm.my\/firdausw\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/people.utm.my\/firdausw\/wp-json\/wp\/v2\/users\/5588"}],"replies":[{"embeddable":true,"href":"https:\/\/people.utm.my\/firdausw\/wp-json\/wp\/v2\/comments?post=406"}],"version-history":[{"count":0,"href":"https:\/\/people.utm.my\/firdausw\/wp-json\/wp\/v2\/posts\/406\/revisions"}],"wp:attachment":[{"href":"https:\/\/people.utm.my\/firdausw\/wp-json\/wp\/v2\/media?parent=406"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/people.utm.my\/firdausw\/wp-json\/wp\/v2\/categories?post=406"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/people.utm.my\/firdausw\/wp-json\/wp\/v2\/tags?post=406"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}