{"id":265,"date":"2012-10-10T08:52:01","date_gmt":"2012-10-10T00:52:01","guid":{"rendered":"http:\/\/comp.utm.my\/azman2\/?page_id=265"},"modified":"2016-04-16T13:51:41","modified_gmt":"2016-04-16T13:51:41","slug":"research-areasinterest","status":"publish","type":"page","link":"https:\/\/people.utm.my\/nurriza\/research-areasinterest\/","title":{"rendered":"Research Interests"},"content":{"rendered":"<p style=\"text-align: justify\"><span style=\"color: #000000\">My main research interest is protein folding which is a study on how amino acid sequence encodes 3D structure of a protein. I am particularly interested in studying the protein folding of human glucose 6-phosphate dehydrogenase (G6PD). G6PD deficiency commonly causes haemolytic disease and is known as the most common enzyme deficiency in humans. Instability of the enzyme has become the centre of attention in G6PD deficiency with the likelihood that a folding defect might be a reason for the lower stability of clinical G6PD variants.<\/span><\/p>\n<p><span style=\"color: #000000\">My research concentrates on:<\/span><br \/>\n<span style=\"color: #000000\"> &#8211; The over-expression of recombinant human G6PD in <em>E. coli<\/em> for structural studies.<\/span><br \/>\n<span style=\"color: #000000\"> &#8211; The refolding of the recombinant human G6PD <em>in vitro.<\/em><\/span><br \/>\n<span style=\"color: #000000\"> &#8211; The solubilisation of inclusion bodies of any recombinant protein and its reactivation.<\/span><br \/>\n<span style=\"color: #000000\"> &#8211; The use of site-directed mutagenesis as a tool in studying protein folding.<\/span><\/p>\n<p style=\"text-align: center\"><span style=\"color: #000000\"><a style=\"color: #000000\" href=\"https:\/\/people.utm.my\/nurriza\/wp-content\/uploads\/sites\/839\/2012\/10\/g6pd.png\"><img loading=\"lazy\" decoding=\"async\" class=\"size-medium wp-image-380 aligncenter\" src=\"https:\/\/people.utm.my\/nurriza\/wp-content\/uploads\/sites\/839\/2012\/10\/g6pd-300x254.png\" alt=\"g6pd\" width=\"300\" height=\"254\" srcset=\"https:\/\/people.utm.my\/nurriza\/wp-content\/uploads\/sites\/839\/2012\/10\/g6pd-300x254.png 300w, https:\/\/people.utm.my\/nurriza\/wp-content\/uploads\/sites\/839\/2012\/10\/g6pd.png 705w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a><\/span><span style=\"color: #000000\">The tetramer of human G6PD (PDB entry code is 1QKI)<\/span><\/p>\n<p style=\"text-align: right\"><em>Science and everyday life cannot and should not be separated<\/em><br \/>\n<em> ~Rosalind Franklin~<\/em><\/p>\n","protected":false},"excerpt":{"rendered":"<p>My main research interest is protein folding which is a study on how amino acid sequence encodes 3D structure of a protein. I am particularly interested in studying the protein folding of human glucose 6-phosphate dehydrogenase (G6PD). G6PD deficiency commonly causes haemolytic disease and is known as the most common enzyme deficiency in humans. Instability [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"open","template":"","meta":{"footnotes":""},"class_list":["post-265","page","type-page","status-publish","hentry","entry"],"_links":{"self":[{"href":"https:\/\/people.utm.my\/nurriza\/wp-json\/wp\/v2\/pages\/265","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/people.utm.my\/nurriza\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/people.utm.my\/nurriza\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/people.utm.my\/nurriza\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/people.utm.my\/nurriza\/wp-json\/wp\/v2\/comments?post=265"}],"version-history":[{"count":0,"href":"https:\/\/people.utm.my\/nurriza\/wp-json\/wp\/v2\/pages\/265\/revisions"}],"wp:attachment":[{"href":"https:\/\/people.utm.my\/nurriza\/wp-json\/wp\/v2\/media?parent=265"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}