International Review of Chemical Engineering
Work on Biopolymer and Biomaterial processing – This study investigated the effects of pH and temperature on the growth and β-glucosidase activity of Lactobacillus rhamnosus NRRL 442 during anaerobic fermentation. Initially, the β-glucosidase activity of the cell harvested from shake flask culture was characterized. The result indicated the cell exhibited the highest specific β-glucosidase activity at pH 6.5 and 46 o C. Subsequently, the effect of fermentation pH (range: 4.5-6.5) on cell growth and β-glucosidase activity was investigated in 2-L bioreactor. Cell suppression due to acidity (pH ≤ 4.5) was observed in fermentation with controlled and uncontrolled pH.
Authors:
Ida Idayu Muhamad, C T Lee, F S Kok & F Razali