About Me

Raihana Ridzuan

Senior Lecturer (DS51)
Department of Biosciences,
Faculty of Science,
Universiti Teknologi Malaysia,
81310 UTM Johor Bahru,
Johor, Malaysia

raihana.r@utm.my

Area of Specialization

  • Plant Breeding and Genetics
  • Plant Tissue Culture
  • Plant Secondary Metabolites

Skill

Laboratory

  • From basic protocol of plant tissue culture of herbs and spices until final stage (acclimatization) of:
  • Herbs: Alpinia conchigera (lengkuas padi), Curcuma manga (temu manga), Andrographis paniculata (hempedu bumi), Etlingera terengganuensis (kantan Terengganu)
  • Orchid:Vanilla planifolia (vanilla orchid), Grammatophyllum speciosum (tiger orchid)
  • Molecular technique
  • DNA and RNA extraction of Capsicum annuum (chili)
  • DNA extraction of fungi, Colletotrichum
  • PCR protocol, gel electrophoresis and viewing
  • Hydro distillation for essential oil extraction
  • Ultra-fast Liquid Chromatography (UFLC) for capsaicinoids determination
  • Total phenolic content and antioxidant assay:
  • 2,2-diphenyl-1-picrylhydrazyl scavenging assay
  • Beta-carotene bleaching assay
  • Antimicrobial technique of herbs and spices against:
  • Species: Escherichia coli, Staphylococcus aureus, Salmonella typhimurium, Bacillus cereus, Aspergillus niger, Candida albicans

Field

  • Fertigation system
  • From irrigation setup until preparation of stock A and B fertilizer
  • Preparation of planting material, layout until harvest

Computer software

  • General: Microsoft Office, Adobe Photoshop, Edraw
  • Statistics: Statistical Package for Social Sciences (SPSS), Statistical Analysis Software (SAS)
  • Genetics: Numerical Taxonomy Multivariate Analysis System (NTSYS), BioEdit, CLUSTAL W2, MEGA 7.