A farewell lunch was held on 10 June 2024 to commemorate Hui Jing’s successful MPhil thesis submission. Hui Jing’s thesis was on “Insight into Microbial Community Dynamics and Methane Metabolism in Palm Oil Mill Effluent Anaerobic Sludge via Next Generation Sequencing”. The project combined temporal sampling of POME anaerobic digester sludge, amplicon, and shotgun metagenomics sequencing to explore further into the intricacies of microbial biomethane production using POME as substrate.
Congratulations Hui Jing and we wish you a success in your future endeavours!

Our group has an opening for an MPhil position on biohydrogen production from pineapple-related wastes, in collaboration with the Sarawak government and Sarawakian agencies and industries. The project will investigate the suitable inocula and substrate for biohydrogen production, before upscaling and optimization in a lab-scale bioreactor. The candidate will be exposed to bioprocess technology, waste treatment, environmental biochemistry, and molecular microbiology.

Monthly allowance will be provided to the successful candidate. Kindly drop me an email at firdausw@utm.my if you are interested.

Candidates from chemistry, biochemistry, biosciences, chemical engineering, bioprocess engineering, and related fields are welcomed to apply. Sarawakian preferred due to the nature of the grant.

Position open until filled.

Feel free to reach out if you are interested in the other sub-projects on bromelain and composting.

Update (12 June 2024): This position has been filled. Thank you for all the interests and I apologise if I missed replying to your messages/emails.

My lab, in collaboration with the Advanced Membrane Technology Research Centre – AMTEC UTM, has an opening for a PhD position on biomethane production performance from palm oil mill effluent (POME). The project will investigate the effects of physicochemical parameters on methane production in a lab-scale bioreactor, and the association with the microbial community dynamics. The candidate will be exposed to bioprocess technology, wastewater treatment, environmental biochemistry, and molecular microbiology.

Monthly allowance will be provided to the successful candidate. Kindly drop me an email at firdausw@utm.my if you are interested.

Candidates from chemistry, biochemistry, biosciences, chemical engineering, bioprocess engineering, and related fields are welcomed to apply. Only open to Malaysians due to the nature of the grant.

Position open until filled.

Our latest review article on the microbial community involved in the conversion of oil palm wastes into biomethane is now published in 3 Biotech! Read the article here.

Abstract
Oil palm processing generates substantial waste materials rich in organic content, posing various environmental challenges. Anaerobic digestion (AD), particularly for palm oil mill effluent (POME), offers a sustainable solution, by converting waste into valuable biomethane for thermal energy or electricity generation. The synergistic activities of the AD microbiota directly affect the biomethane production, and the microbial community involved in biomethane production in POME anaerobic digestion has been reported. The composition of bacterial and archaeal communities varies under different substrate and physicochemical conditions. This review discusses the characteristics of POME, explores the microbial members engaged in each stage of AD, and elucidates the impacts of substrate and physicochemical conditions on the microbial community dynamics, with a specific focus on POME. Finally, the review outlines current research needs and provides future perspectives on optimizing the microbial communities for enhanced biomethane production from oil palm wastes.

Our recent preliminary results on the investigation of the POME anaerobic digester microbiomes are now published in the South Asian Journal of Research in Microbiology (Open Access). Check out the full paper here.

Abstract
Palm Oil Mill Effluents (POME) serve as suitable substrates for methane gas production through anaerobic digestion. This process relies on a complex microbial community that plays a critical role in ensuring stable anaerobic digester operation and efficient biogas production. Among these microorganisms, methanogenic archaea are pivotal in methane generation by utilizing diverse substrates under anoxic conditions. However, the knowledge of the microbial communities, particularly those involved in methane production in POME anaerobic sludge at different time intervals, remains limited. This study aims to uncover temporal variations in microbial communities, including diversity, composition, and structure, within POME anaerobic sludge, specifically focusing on the methanogenic archaea community. The temporal dynamics of microbial communities in the eighteen POME anaerobic sludge samples collected from a palm oil mill were investigated through 16S rRNA amplicon sequencing. The results reveal consistent microbial community diversity in POME anaerobic sludge over the study periods. Then, the sequencing also showed that Bacillota (26.9 ± 3.3%), Bacteroidota (20.2 ± 5.3%), and Chloroflexota (15.0% ± 6.3%) were the dominant bacterial phyla in POME anaerobic sludge across different time frames. Concurrently, Halobacteriota (5.9 ± 2.8%), Methanobacteriota (2.5 ± 0.6%), and Nanoarchaeota (2.3 ± 1.2%) were the primary archaeal phyla identified in anaerobic sludge at various time intervals. Furthermore, amplicon sequencing revealed the presence of two methanogenic archaea genera, Methanothrix and Methanobacterium, associated with acetoclastic and hydrogenotrophic methanogenesis, respectively. These findings suggest that acetoclastic and hydrogenotrophic methanogenesis pathways are the primary contributors to methane production in the POME anaerobic digestion process.

An article by the lab members has been published in the UTMNexus magazine (April 2023 issue). UTMNexus is a publication coordinated by UTM DVCRI office, targeted at the general public, showcasing the state-of-the-art research conducted by the university researchers. Our article describes the role of microorganisms in climate change and energy security, in line with the theme of this issue. We introduced the readers to the characteristics and biotechnological potentials of archaea, which is among the least understood groups of microbes, in carbon capture and conversion. The article was also co-authored by our collaborators from PETRONAS Research Sdn. Bhd. 

Read the article here: https://indd.adobe.com/view/c3f850ba-3dcf-4368-b682-ab813e43932d
Find out more about UTMNexus: https://research.utm.my/magazine/

Eka Latiffah Nadia Dzulkarnain recently submitted her final hardbound Master of Philosophy (MPhil) thesis. Eka’s research project was on the effects of different heat pretreatment temperatures on biohydrogen production using inoculum from anaerobic sludge. She also investigated the changes caused by the pretreatment to the microbial community dynamics and functional potentials using metagenomics. We wish her all the best in all her future endeavours!

Julia Ameera Johari bagged the first prize in the Biosciences 3-Minute Presentation (3MP) Competition held at the Faculty of Science recently. Julia is working on her Final Year Undergraduate Project in the lab, investigating the isotopic methane production from methanogenic microbial community in anaerobic sludge. Congratulations Julia!

A coffee session with the lab members was held on 14 December 2022, to commemorate Eka’s successful defence of her MPhil thesis on 13 December 2022. It was good to talk science and life over coffee and cakes!

I was grateful to be given the opportunity to contribute to the Science Diplomacy magazine, which is a publication by the National Institute of Science Communication and Information Resources, India. I took the opportunity to write about my personal journey to science diplomacy, starting with my interest in international affairs and relations, to my participation in the TWAS-AAAS Course on Science Diplomacy in Trieste, Italy.
Read about it here (open-access). Click here for the Science Diplomacy magazine page.